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Item #:
148019655
AA12S05-2546WT Reversed Phase C18 Column, ODS-A, 120 Angstrom, 5 Micrometers, 250mm x 4.6mm
92% of respondents would recommend this to a friend
Item #:
148019655
BND 1678
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Order now and get it around Monday, November 02
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The ODS-A column from YMC America offers selectivity in a wide range of organic compounds, making it ideal for the pharmaceutical, chemical, and biotechnology industries.
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What Stands Out
High Efficiency
The 5-micron particle size delivers exceptional separation and resolution, catering to demanding analytical applications in pharmaceuticals and research industries.
Versatile Applications
Designed for versatile use in reversed-phase liquid chromatography, this column effectively addresses diverse analytical challenges across various sample types.
Durable Design
Built to withstand rigorous conditions, the 120 Angstrom pore size ensures optimal performance and longevity, providing reliable results over extended use.
Product Details
Product Technical Details
| Package Dimensions | 16 x 3 x 2 inches |
| Manufacturer | Thomas Scientific |
| Item model number | AA12S05-2546WT |
Who Should Buy?
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Analytical Chemists
Ideal for analytical testing of small molecules in various applications like pharmaceuticals and environmental analysis.
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Laboratory Researchers
Perfect for research settings requiring high resolution chromatography for method development and validation.
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Quality Control
Suitable for routine quality control in labs needing dependable and consistent separation of complex mixtures.
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Large Sample Volumes
Not suitable for applications involving large sample volumes as its capacity may lead to poor performance.
Product Description
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Customer Questions & Answers
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Question:
What is the primary application of the AA12S05-2546WT Reversed Phase C18 Column?
Answer: The AA12S05-2546WT Reversed Phase C18 Column is primarily used for high-performance liquid chromatography (HPLC) applications. It is designed for the separation of a wide range of organic compounds, making it ideal for pharmaceutical analysis, environmental testing, and food safety evaluations. This versatility is due to its stationary phase of C18 material, which effectively retains non-polar molecules while allowing polar compounds to elute quickly. -
Question:
What are the specifications of the AA12S05-2546WT column?
Answer: The AA12S05-2546WT column features dimensions of 250mm x 4.6mm with a particle size of 5 micrometers and a pore size of 120 angstroms. These specifications ensure optimal flow rates and resolution during chromatographic processes, which is crucial for achieving reproducible results in analytical applications. Its 5-micrometer particles enhance the surface area for interactions, resulting in improved separation efficiency. -
Question:
How does the reversed phase function in chromatography?
Answer: In reversed phase chromatography, the stationary phase is non-polar while the mobile phase is polar. This setup allows for better retention of non-polar compounds, which are forced to interact with the column material. As a result, analytes with varying polarities can be effectively separated based on their hydrophobic properties. This principle is particularly useful in separating complex mixtures, such as in pharmaceutical development or environmental samples. -
Question:
Can the AA12S05-2546WT C18 column be used for protein analysis?
Answer: While the AA12S05-2546WT C18 column is optimized for the separation of small organic molecules, it is not typically recommended for protein analysis due to the potential for denaturation. For proteins and larger biomolecules, dedicated columns designed specifically for such applications should be used. However, it can still be utilized for small peptides and low molecular weight compounds in protein research settings. -
Question:
What types of mobile phases are suitable for use with this column?
Answer: Suitable mobile phases for the AA12S05-2546WT column include a range of polar solvents, such as water, methanol, acetonitrile, or their mixtures containing buffers. The choice of mobile phase will depend on the nature of the analyte and desired separation characteristics. Experimenting with different solvent compositions can yield optimal results, particularly in adapting to the specific needs of chromatographic methods. -
Question:
What are some recommended practices for maintaining the AA12S05-2546WT column?
Answer: To maintain the AA12S05-2546WT column, it's vital to use well-filtered and degassed solvents to prevent particulate contamination. Regularly flushing the column with a suitable cleaning solvent when not in use will help preserve its performance. Additionally, monitoring pressure and ensuring that operational conditions are within specified limits can prevent damage and prolong the lifespan of the column. -
Question:
Is the AA12S05-2546WT column compatible with different HPLC systems?
Answer: Yes, the AA12S05-2546WT column is compatible with various HPLC systems that support standard fitting sizes. This adaptability allows researchers and analysts to integrate it into their established workflows. Whether you're conducting routine quality control or complex research applications, its versatility ensures consistent performance across different instrumentation setups. -
Question:
What is the optimal temperature range for using the AA12S05-2546WT column?
Answer: The optimal temperature range for using the AA12S05-2546WT column typically lies between 25°C and 60°C, depending on the specific application and solvent system. Operating within this range can improve peak shapes and reduce analysis times by enhancing mass transfer rates. High temperatures may also facilitate the separation of challenging analytes, but care should be taken to avoid damage to the column. -
Question:
What should I consider when selecting a C18 column for my application?
Answer: When selecting a C18 column like the AA12S05-2546WT for your application, consider the nature of your analytes, the expected separation efficiency, and compatibility with your HPLC system. Factors such as particle size, pore size, and column dimensions also play a crucial role in achieving desired resolution and retention times. Tailoring these parameters to your specific analytical needs will ensure optimal performance. -
Question:
Where can I buy the AA12S05-2546WT Reversed Phase C18 Column?
Answer: You can purchase the AA12S05-2546WT Reversed Phase C18 Column from Ubuy in Brunei. Ubuy offers a range of laboratory supplies and equipment, ensuring that you receive quality products for your chromatographic applications.
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BND 1678
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Features & Benefits
- End-capped C18 phase for enhanced selectivity.
- Designed for reversed-phase separation.
- Perfect for pharmaceutical, chemical, and biotechnology applications.
- Features a 5 micrometer particle size with 120 angstrom porosity.
- Dimensions: 4.6mm ID and 250mm length.
- Manufactured in the USA for quality assurance.
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